Journal: bioRxiv

Article Title: Microsecond pulse electrical stimulation modulates cell migration

doi: 10.1101/2022.10.23.513372

Figure Lengend Snippet: (a) A graphical illustration showed the effects of μsPEF (pulse width:20 μs, frequency: 10 Hz, duration: 5 s) on the skin wound, promoting cell migration and extracellular matrix remodeling. (b) Representative time-lapse images showing the fibroblasts morphology after μsPEF (i.e., 750 and 1500 V/cm) treatment of different intensity at different time points (i.e., 0, 1 and 2 h). Detached cells are marked by yellow arrow. Scale bar, 50 μm. (c) The line graph representing the cell migration average speed per 1 h from 0 to 12 h after different intensity μsPEF (i.e., 750 and 1500 V/cm) treatment (blue circle: control; orange square: 750 V/cm; pink triangle: 1500 V/cm). Results are presented as mean ± standard deviation with 95% CI (n CTRL =42 cells, n 750 v/cm =48 cells, n 1500 v/cm =47 cells); * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 versus control by one-way ANOVA for multiple comparisons. (d) Box plot showing the average cell migration speed with different intensity μsPEF (i.e., 750 and 1500 V/cm) and control treatments in 24 h. Results are presented as mean ± standard deviation with 95% CI (n CTRL =470 cells, n 750 V/cm =979 cells, n 1500 V/cm =535 cells). (e) Effects of μsPEF on the secretion of COLA2. The level of collagen type I α2 in cellular supernatants was measured after 48 h. The concentration of COLA2 was 0.109 ± 0.018 ng/mL in the control group, 0.257 ± 0.058 ng/mL in the 750 V/cm group and 0.363 ± 0.034 ng/mL in the 1500 V/cm group. Results are presented as mean ± standard deviation with 95% CI (n=5); * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 versus control by one-way ANOVA for multiple comparisons. (f) Effects of μsPEF on the expression of FGF2. The level of FGF2 in cellular supernatants was measured after 48 h. The concentration of FGF2 was 44.139 ± 0.360 ng/mL in the control group, 48.012 ± 1.488 ng/mL in the 750 V/cm group and 48.523 ± 1.944 ng/mL in the 1500 V/cm group. Results are presented as mean ± standard deviation with 95% CI (n=3); ns=0.7329, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 versus control by one-way ANOVA for multiple comparisons.

Article Snippet: Then, the cells were cultured in serum-free medium for 48 h. The content of type I α collagen and basic fibroblast growth factor (FGF-2) in the supernatant were measured using commercially available Human COL1A2 (Collagen Type I Alpha 2) ELISA Kit (Elabscience, Wuhan, China) and Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit (Elabscience, Wuhan, China) according to the manufacturer’s protocol respectively.

Techniques: Migration, Control, Standard Deviation, Concentration Assay, Expressing

Journal: Theranostics

Article Title: Nanodelivery of Y-27632 by RGD-modified liposome enhances radioimmunotherapy of hepatocellular carcinoma via tumor microenvironment matrix stiffness reprogramming

doi: 10.7150/thno.114892

Figure Lengend Snippet: Preparation and characterization of RGD@LP-Y. ( A ) Schematic illustration of RGD@LP-Y preparation. ( B ) TEM observation of RGD@LP-Y morphology. ( C ) Zeta potential of RGD@LP-Y. ( D ) The cumulative release of LP-Y and RGD@LP-Y at different pH values (pH 7.4 and 5.5). ( E ) Variations in the particle size of RGD@LP-Y under conditions of 4 ℃, -20 ℃, -80 ℃, and 10% serum after 12 days. ( F ) Relative viability of Hepa1-6 cells treated with different liposomes measured by CCK-8 assay. ( G ) mRNA expression levels of YAP and COL1A2. ( F ) Detection of COL1 level in Hepa1-6 cell supernatant by ELISA. LP, liposomes without RGD modification; RGD@LP, RGD-modified liposomes; LP-Y, liposomes encapsulating Y-27632 but without RGD modification; RGD@LP-Y, RGD-modified liposomes encapsulated with Y-27632. Values represent mean±SD. *P < 0.05, **P < 0.01, ***P < 0.001. ns: not significant.

Article Snippet: The Mouse IL-12 (Interleukin 12) ELISA Kit [E-EL-M3062], Mouse COL1 (Collagen Type I) ELISA Kit[E-EL-M0325], Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit [E-EL-H0109], Human IL-6 (Interleukin 6) ELISA Kit [E-EL-H6156], Human IL-1β (Interleukin 1 Beta) ELISA Kit [E-EL-H0149], Mouse IL-6 (Interleukin 6) ELISA Kit [E-EL-M0044], and Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit [E-EL-M3063] were purchased from Elabscience Biotechnology Co., Ltd. (China).

Techniques: Zeta Potential Analyzer, Liposomes, CCK-8 Assay, Expressing, Enzyme-linked Immunosorbent Assay, Modification

Journal: Scientific Reports

Article Title: IGF-1 regulates LARP6-mediated collagen metabolism in vaginal fibroblasts of POP patients via the PI3K/AKT pathway

doi: 10.1038/s41598-025-08509-x

Figure Lengend Snippet: IHC for COL1, COL3, IGF-1, LARP6 and MMP2 in the anterior vaginal wall.

Article Snippet: The following were the relevant antibodies: i. collagen type I (COL1) antibody (1/100, CY5120, Abways), ii. collagen type III (COL3) antibody (1/1000, 22734-1-AP, Proteintech), iii.

Techniques:

Journal: Scientific Reports

Article Title: IGF-1 regulates LARP6-mediated collagen metabolism in vaginal fibroblasts of POP patients via the PI3K/AKT pathway

doi: 10.1038/s41598-025-08509-x

Figure Lengend Snippet: RT-qPCR detection of COL1, COL3, MMP2 and LARP6 gene expression and COL1 to COL3 gene expression ratio statistics.

Article Snippet: The following were the relevant antibodies: i. collagen type I (COL1) antibody (1/100, CY5120, Abways), ii. collagen type III (COL3) antibody (1/1000, 22734-1-AP, Proteintech), iii.

Techniques: Quantitative RT-PCR, Gene Expression

Journal: Scientific Reports

Article Title: IGF-1 regulates LARP6-mediated collagen metabolism in vaginal fibroblasts of POP patients via the PI3K/AKT pathway

doi: 10.1038/s41598-025-08509-x

Figure Lengend Snippet: Protein expression of COL1, COL3, LARP6 and p-AKT and AKT and COL1/COL3 expression statistics.

Article Snippet: The following were the relevant antibodies: i. collagen type I (COL1) antibody (1/100, CY5120, Abways), ii. collagen type III (COL3) antibody (1/1000, 22734-1-AP, Proteintech), iii.

Techniques: Expressing

Journal: Scientific Reports

Article Title: IGF-1 regulates LARP6-mediated collagen metabolism in vaginal fibroblasts of POP patients via the PI3K/AKT pathway

doi: 10.1038/s41598-025-08509-x

Figure Lengend Snippet: Expression of mRNAs for COL1, COL3, LARP6 and p-AKT and AKT and COL1/COL3 expression statistics.

Article Snippet: The following were the relevant antibodies: i. collagen type I (COL1) antibody (1/100, CY5120, Abways), ii. collagen type III (COL3) antibody (1/1000, 22734-1-AP, Proteintech), iii.

Techniques: Expressing

Journal: Scientific Reports

Article Title: IGF-1 regulates LARP6-mediated collagen metabolism in vaginal fibroblasts of POP patients via the PI3K/AKT pathway

doi: 10.1038/s41598-025-08509-x

Figure Lengend Snippet: Protein expression of LARP6, COL1 and COL3 and statistics of the ratio of COL1 to COL3 protein expression.

Article Snippet: The following were the relevant antibodies: i. collagen type I (COL1) antibody (1/100, CY5120, Abways), ii. collagen type III (COL3) antibody (1/1000, 22734-1-AP, Proteintech), iii.

Techniques: Expressing

Journal: Scientific Reports

Article Title: IGF-1 regulates LARP6-mediated collagen metabolism in vaginal fibroblasts of POP patients via the PI3K/AKT pathway

doi: 10.1038/s41598-025-08509-x

Figure Lengend Snippet: Expression of LARP6, COL1 and COL3 mRNAs and the ratio of COL1 to COL3 gene expression statistics.

Article Snippet: The following were the relevant antibodies: i. collagen type I (COL1) antibody (1/100, CY5120, Abways), ii. collagen type III (COL3) antibody (1/1000, 22734-1-AP, Proteintech), iii.

Techniques: Expressing, Gene Expression

Journal: Journal of diabetes research

Article Title: Exendin-4 Improves Diabetic Kidney Disease in C57BL/6 Mice Independent of Brown Adipose Tissue Activation.

doi: 10.1155/2020/9084567

Figure Lengend Snippet: Figure 2: Exendin-4 ameliorated the progress of diabetic nephropathy and activated the renal AMPK pathway. The 24-hour urinary albumin (a) and 24-hour urinary 8-OH-dG (b) were measured by ELISA. (c) Periodic acid-Schiff(PAS) and Masson staining of kidney. Magnification ×400. (d–f) The renal mRNA expression of TGF-β1, Col-1, and FN, respectively (n = 6 per group). Western blot analysis of renal TGF-β1 (g, h) and AMPK (i, j) protein and the quantitative analysis results (n = 5 per group). n = 6~8 per group. ∗P < 0:05, ∗∗P < 0:01, and ∗∗∗P < 0:001 vs. control group; #P < 0:05, ##P < 0:01, and ###P < 0:001 vs. DM+Exc group; &P < 0:05, &&P < 0:01, and &&&P < 0:001 vs. DM-Con group.

Article Snippet: The primary antibodies were as follows: phosphoadenosine 5′-monophosphate- (AMP-) activated protein kinase (AMPK) α (Thr172) and AMPKα, phospho-acetylCoA carboxylase (ACC) (Ser79) and ACC, transforming growth factor- (TGF-) β1 (all from Cell Signaling Technology, Danvers, MA, USA), collagen I (Col-1) and fibronectin (FN) (both from Abcam, Cambridge, MA, USA), and αsmooth muscle actin (SMA) and β-actin (both from Boster Bio Co., Wuhan, China). nloaded from https://onlinelibrary.w iley.com /doi/10.1155/2020/9084567 by U niversity O f T he Philippines, W iley O nline L ibrary on [13/10/2024].

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Control